Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices

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Update on Cyclospora cayetanensis, a food-borne and waterborne parasite.

The coccidian parasite Cyclospora cayetanensis is recognized as an emerging pathogen that causes protracted diarrhea in humans. The first cases of Cyclospora infection were reported in the late 1970s and were observed among expatriates and travelers in regions where infections are endemic. Since then, Cyclospora has been considered a cause of traveler's diarrhea. Epidemiological investigations ...

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Emerging pathogen Cyclospora cayetanensis infection in Nepal.

Cylospora cayetanensis, an emerging parasitic pathogen of human is being increasingly recognized throughout the world, however the means of transmission and the possibility of a reservoir host remain an enigma. A longitudinal study on cyclosporiasis in different parts of Nepal was carried out from April, 1995 until November, 2000. Fecal specimens were collected from symptomatic and asymptomatic...

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Epidemiologic studies of Cyclospora cayetanensis in Guatemala.

In 1996 and 1997, cyclosporiasis outbreaks in North America were linked to eating Guatemalan raspberries. We conducted a study in health-care facilities and among raspberry farm workers, as well as a case-control study, to assess risk factors for the disease in Guatemala. From April 6, 1997, to March 19, 1998, 126 (2.3%) of 5, 552 surveillance specimens tested positive for Cyclospora; prevalenc...

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Implications of culture-independent panel-based detection of Cyclospora cayetanensis.

The Nebraska Medical Center (TNMC) was conducting an Institutional Review Board 8 approved research study utilizing the BioFire FilmArray Gastrointestinal Panel (BioFire 9 Diagnostics, Salt Lake City, UT) when an outbreak of cyclosporiasis occurred in Iowa and 10 Nebraska (1). The FilmArray GI Panel is a culture-independent molecular test that 11 simultaneously detects 14 bacterial, 5 viral, an...

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Multilocus Sequence Typing Tool for Cyclospora cayetanensis

Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.

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ژورنال

عنوان ژورنال: Food and Waterborne Parasitology

سال: 2021

ISSN: 2405-6766

DOI: 10.1016/j.fawpar.2021.e00111